primary human nucleus pulposus (np) cells Search Results


human nucleus pulposus (np, cat. no. 4800) cells  (ScienCell)
90
ScienCell human nucleus pulposus (np, cat. no. 4800) cells
LPS-induced <t>nucleus</t> <t>pulposus</t> cell apoptosis is inhibited by miR-222 inhibitor. (A) miR-222 level in human intervertebral disc tissues was detected by reverse transcription-quantitative polymerase chain reaction. (B) miR-222 level in lipopolysaccharide-stimulated nucleus pulposus cells was detected using reverse transcription-quantitative polymerase chain reaction. (C) Transfection efficiency of miR-222 mock, mimics and inhibitor was determined by reverse transcription-quantitative polymerase chain reaction. (D) Nucleus pulposus cell apoptosis was analyzed by flow cytometry. * P<0.05 and ** P<0.01 vs. control; # P<0.05 and ## P<0.01 vs. cells stimulated with LPS only. LPS, lipopolysaccharide.
Human Nucleus Pulposus (Np, Cat. No. 4800) Cells, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+nucleus+pulposus+(np)+cells/Human+nucleus+pulposus++NP++cat++no++4800++cells/pmc06713428-72-0-11
Average 90 stars, based on 1 article reviews
human nucleus pulposus (np, cat. no. 4800) cells - by Bioz Stars, 2026-09
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primary human nucleus pulposus cells (npcs, cat. no. csi113hu01)  (USCN Life)
90
USCN Life primary human nucleus pulposus cells (npcs, cat. no. csi113hu01)
LPS-induced <t>nucleus</t> <t>pulposus</t> cell apoptosis is inhibited by miR-222 inhibitor. (A) miR-222 level in human intervertebral disc tissues was detected by reverse transcription-quantitative polymerase chain reaction. (B) miR-222 level in lipopolysaccharide-stimulated nucleus pulposus cells was detected using reverse transcription-quantitative polymerase chain reaction. (C) Transfection efficiency of miR-222 mock, mimics and inhibitor was determined by reverse transcription-quantitative polymerase chain reaction. (D) Nucleus pulposus cell apoptosis was analyzed by flow cytometry. * P<0.05 and ** P<0.01 vs. control; # P<0.05 and ## P<0.01 vs. cells stimulated with LPS only. LPS, lipopolysaccharide.
Primary Human Nucleus Pulposus Cells (Npcs, Cat. No. Csi113hu01), supplied by USCN Life, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+nucleus+pulposus+(np)+cells/primary+human+nucleus+pulposus+cells++NPCs++cat++no++CSI113Hu01/pm36301028-39-11-23
Average 90 stars, based on 1 article reviews
primary human nucleus pulposus cells (npcs, cat. no. csi113hu01) - by Bioz Stars, 2026-09
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primary human nucleus pulposus (np) cells  (iCell Gene Therapeutics)
90
iCell Gene Therapeutics primary human nucleus pulposus (np) cells
LPS-induced <t>nucleus</t> <t>pulposus</t> cell apoptosis is inhibited by miR-222 inhibitor. (A) miR-222 level in human intervertebral disc tissues was detected by reverse transcription-quantitative polymerase chain reaction. (B) miR-222 level in lipopolysaccharide-stimulated nucleus pulposus cells was detected using reverse transcription-quantitative polymerase chain reaction. (C) Transfection efficiency of miR-222 mock, mimics and inhibitor was determined by reverse transcription-quantitative polymerase chain reaction. (D) Nucleus pulposus cell apoptosis was analyzed by flow cytometry. * P<0.05 and ** P<0.01 vs. control; # P<0.05 and ## P<0.01 vs. cells stimulated with LPS only. LPS, lipopolysaccharide.
Primary Human Nucleus Pulposus (Np) Cells, supplied by iCell Gene Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+nucleus+pulposus+(np)+cells/primary+human+nucleus+pulposus++np++cells/ppr0904741-58-3-13
Average 90 stars, based on 1 article reviews
primary human nucleus pulposus (np) cells - by Bioz Stars, 2026-09
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human nucleus pulposus np cells human annulus fibrosus af cells non magnetic microcubes  (Orthofix Medical Inc)
86
Orthofix Medical Inc human nucleus pulposus np cells human annulus fibrosus af cells non magnetic microcubes
LPS-induced <t>nucleus</t> <t>pulposus</t> cell apoptosis is inhibited by miR-222 inhibitor. (A) miR-222 level in human intervertebral disc tissues was detected by reverse transcription-quantitative polymerase chain reaction. (B) miR-222 level in lipopolysaccharide-stimulated nucleus pulposus cells was detected using reverse transcription-quantitative polymerase chain reaction. (C) Transfection efficiency of miR-222 mock, mimics and inhibitor was determined by reverse transcription-quantitative polymerase chain reaction. (D) Nucleus pulposus cell apoptosis was analyzed by flow cytometry. * P<0.05 and ** P<0.01 vs. control; # P<0.05 and ## P<0.01 vs. cells stimulated with LPS only. LPS, lipopolysaccharide.
Human Nucleus Pulposus Np Cells Human Annulus Fibrosus Af Cells Non Magnetic Microcubes, supplied by Orthofix Medical Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+human+nucleus+pulposus+(np)+cells/af+annulus+cells+cells+fibrosus+human+human+magnetic+microcubes+non+np+nucleus+pulposus/pinney_james_richardson__2014__development_and_implementation_of_discrete_polymeric_microstructural_cues_for_applications_in-842-5-4
Average 86 stars, based on 1 article reviews
human nucleus pulposus np cells human annulus fibrosus af cells non magnetic microcubes - by Bioz Stars, 2026-09
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LPS-induced nucleus pulposus cell apoptosis is inhibited by miR-222 inhibitor. (A) miR-222 level in human intervertebral disc tissues was detected by reverse transcription-quantitative polymerase chain reaction. (B) miR-222 level in lipopolysaccharide-stimulated nucleus pulposus cells was detected using reverse transcription-quantitative polymerase chain reaction. (C) Transfection efficiency of miR-222 mock, mimics and inhibitor was determined by reverse transcription-quantitative polymerase chain reaction. (D) Nucleus pulposus cell apoptosis was analyzed by flow cytometry. * P<0.05 and ** P<0.01 vs. control; # P<0.05 and ## P<0.01 vs. cells stimulated with LPS only. LPS, lipopolysaccharide.

Journal: International Journal of Molecular Medicine

Article Title: Knockdown of miR-222 inhibits inflammation and the apoptosis of LPS-stimulated human intervertebral disc nucleus pulposus cells

doi: 10.3892/ijmm.2019.4314

Figure Lengend Snippet: LPS-induced nucleus pulposus cell apoptosis is inhibited by miR-222 inhibitor. (A) miR-222 level in human intervertebral disc tissues was detected by reverse transcription-quantitative polymerase chain reaction. (B) miR-222 level in lipopolysaccharide-stimulated nucleus pulposus cells was detected using reverse transcription-quantitative polymerase chain reaction. (C) Transfection efficiency of miR-222 mock, mimics and inhibitor was determined by reverse transcription-quantitative polymerase chain reaction. (D) Nucleus pulposus cell apoptosis was analyzed by flow cytometry. * P<0.05 and ** P<0.01 vs. control; # P<0.05 and ## P<0.01 vs. cells stimulated with LPS only. LPS, lipopolysaccharide.

Article Snippet: Human nucleus pulposus (NP, cat. no. 4800) cells were obtained from ScienCell Research Laboratories and cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco; Thermo Fisher Scientific) containing 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific) and antibiotics (1% penicillin/streptomycin, BBI Life Sciences) at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Transfection, Flow Cytometry, Control

Effects of miR-222 on TNF-α, IL-1β and IL-6, collagen II and aggrecan expression levels in LPS-treated nucleus pulposus cells. (A) TNF-α, (B) IL-1β and (C) IL-6 expression levels in nucleus pulposus cells treated with LPS were detected by ELISA. The expression levels of (D) collagen II and (E) aggrecan in nucleus pulposus cells treated with LPS were detected using reverse transcription-quantitative polymerase chain reaction * P<0.05 and ** P<0.01 vs. control, # P<0.05 and ## P<0.01 vs. cells stimulated with LPS only. TNF-α, tumor necrosis factor- α; IL, interleukin; LPS, lipopolysaccharide.

Journal: International Journal of Molecular Medicine

Article Title: Knockdown of miR-222 inhibits inflammation and the apoptosis of LPS-stimulated human intervertebral disc nucleus pulposus cells

doi: 10.3892/ijmm.2019.4314

Figure Lengend Snippet: Effects of miR-222 on TNF-α, IL-1β and IL-6, collagen II and aggrecan expression levels in LPS-treated nucleus pulposus cells. (A) TNF-α, (B) IL-1β and (C) IL-6 expression levels in nucleus pulposus cells treated with LPS were detected by ELISA. The expression levels of (D) collagen II and (E) aggrecan in nucleus pulposus cells treated with LPS were detected using reverse transcription-quantitative polymerase chain reaction * P<0.05 and ** P<0.01 vs. control, # P<0.05 and ## P<0.01 vs. cells stimulated with LPS only. TNF-α, tumor necrosis factor- α; IL, interleukin; LPS, lipopolysaccharide.

Article Snippet: Human nucleus pulposus (NP, cat. no. 4800) cells were obtained from ScienCell Research Laboratories and cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco; Thermo Fisher Scientific) containing 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific) and antibiotics (1% penicillin/streptomycin, BBI Life Sciences) at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Control

Overexpression of TIMP3 reverses the apoptosis of LPS-stimulated nucleus pulposus cells induced by miR-222 inhibitor. (A) TIMP3 mRNA levels was determined by reverse transcription-quantitative polymerase chain reaction. (B) TIMP3 protein levels were determined by western blot analysis. (C) The relative TIMP3 protein level is presented. (D) Cell apoptosis was analyzed by flow cytometry. ** P<0.01 vs. control; ## P<0.01 vs. cells stimulated with LPS only; && P<0.01 vs. inhibitor + siNC.

Journal: International Journal of Molecular Medicine

Article Title: Knockdown of miR-222 inhibits inflammation and the apoptosis of LPS-stimulated human intervertebral disc nucleus pulposus cells

doi: 10.3892/ijmm.2019.4314

Figure Lengend Snippet: Overexpression of TIMP3 reverses the apoptosis of LPS-stimulated nucleus pulposus cells induced by miR-222 inhibitor. (A) TIMP3 mRNA levels was determined by reverse transcription-quantitative polymerase chain reaction. (B) TIMP3 protein levels were determined by western blot analysis. (C) The relative TIMP3 protein level is presented. (D) Cell apoptosis was analyzed by flow cytometry. ** P<0.01 vs. control; ## P<0.01 vs. cells stimulated with LPS only; && P<0.01 vs. inhibitor + siNC.

Article Snippet: Human nucleus pulposus (NP, cat. no. 4800) cells were obtained from ScienCell Research Laboratories and cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco; Thermo Fisher Scientific) containing 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific) and antibiotics (1% penicillin/streptomycin, BBI Life Sciences) at 37°C in a humidified atmosphere of 5% CO 2 .

Techniques: Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Flow Cytometry, Control